GSE263777 Two distinct Trypanosoma eIF4F complexes co-exist, bind different mRNAs and are regulated during nutritional stress

Contributors : Bernardo Papini Gabiatti ; Eden Ribeiro Freire ; Jimena Ferreira da Costa ; Mariana Galv ão Ferrarini ; Tatiana Reichert Assunção de Matos ; Henrique Preti ; Isadora Munhoz da Rocha ; Beatriz Gomes Guimarães ; Susanne Kramer ; Nilson Ivo Tonin Zanchin ; Fabíola Barbieri HoletzSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Trypanosoma cruziMany eIF4F subunits and PABP paralogues are found in trypanosomes: six eIF4E, five eIF4G, one eIF4A and two PABPs. They are expressed simultaneously and assemble into different complexes, contrasting the situation in metazoans that use distinct complexes in different cell types or developmental stages. Each eIF4F complex has its own proteins, mRNAs and, consequently, a distinct function. We set out to study the function and regulation of the two major eIF4F complexes of Trypanosoma cruzi. We identified the associated proteins and mRNAs of eIF4E3 and eIF4E4 in cells in exponential growth and in nutritional stress. Upon stress, eIF4G/eIF4A and PABP remain associated to the eIF4E, but the association with other 43S pre-initiation factors decreases, indicating that ribosome attachment is impaired. Most eIF4E3-associated mRNAs encode for metabolic proteins, while eIF4E4 associate to mRNAs encoding ribosomal proteins. Interestingly, for both eIF4E3/4, more mRNAs were associated in stressed cells than in non-stressed cells, even though stress causes ribosome disassembly. In summary,...

Source: GEO: Gene Expression Omnibus - April 14, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Trypanosoma cruzi Source Type: research