Femtosecond optical studies of the primary charge separation reactions in far-red photosystem II from Synechococcus sp. PCC 7335

Biochim Biophys Acta Bioenerg. 2024 Apr 6:149044. doi: 10.1016/j.bbabio.2024.149044. Online ahead of print.ABSTRACTPrimary processes of light energy conversion by Photosystem II (PSII) were studied using femtosecond broadband pump-probe absorption difference spectroscopy. Transient absorption changes of core complexes isolated from the cyanobacterium Synechococcus sp. PCC 7335 grown under far-red light (FRL-PSII) were compared with the canonical Chl a containing spinach PSII core complexes upon excitation into the red edge of the Qy band. Absorption changes of FRL-PSII were monitored at 278 K in the 400-800 nm spectral range on a timescale of 0.1-500 ps upon selective excitation at 740 nm of four chlorophyll (Chl) f molecules in the light harvesting antenna, or of one Chl d molecule at the ChlD1 position in the reaction center (RC) upon pumping at 710 nm. Numerical analysis of absorption changes and assessment of the energy levels of the presumed ion-radical states made it possible to identify PD1+ChlD1- as the predominant primary charge-separated radical pair, the formation of which upon selective excitation of Chl d has an apparent time of ~1.6 ps. Electron transfer to the secondary acceptor pheophytin PheoD1 has an apparent time of ~7 ps with a variety of excitation wavelengths. The energy redistribution between Chl a and Chl f in the antenna occurs within 1 ps, whereas the energy migration from Chl f to the RC occurs mostly with lifetimes of 60 and 400 ps. Potentiometric ...

Source: Biochimica et Biophysica Acta - April 8, 2024 Category: Biochemistry Authors: Dmitry A Cherepanov Vasily Kurashov Fedor E Gostev Ivan V Shelaev Alexey A Zabelin Gaozhong Shen Mahir D Mamedov Arseny Aybush Anatoly Ya Shkuropatov Victor A Nadtochenko Donald A Bryant John H Golbeck Alexey Yu Semenov Source Type: research