Copper-mediated siRNA activation for conditional control of gene expression

We present a chemical approach of selective siRNA activation triggered by intracellular copper ions. We designed and synthesized nucleotides containing copper-responsive moieties, which were incorporated into siRNAs. These copper-responsive siRNAs effectively silenced the target cyclin B1 mRNA in living cells. This pioneering study introduces a novel method for conditionally controlling gene silencing using biologically relevant metal ions in human cells, thereby expanding the repertoire of chemical knockdown tools.PMID:38593925 | DOI:10.1016/j.bmcl.2024.129738
Source: Bioorganic and Medicinal Chemistry Letters - Category: Chemistry Authors: Source Type: research