Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP)

Adv Exp Med Biol. 2024;3234:1-15. doi: 10.1007/978-3-031-52193-5_1.ABSTRACTThroughout their life cycle, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Each mRNA is part of multiple successive mRNP complexes that participate in their biogenesis, cellular localization, translation and decay. The dynamic composition of mRNP complexes and their structural remodelling play crucial roles in the control of gene expression. Studying the endogenous composition of different mRNP complexes is a major challenge. In this chapter, we describe the variety of protein-centric immunoprecipitation methods available for the identification of mRNP complexes and the requirements for their experimental settings.PMID:38507196 | DOI:10.1007/978-3-031-52193-5_1

Source: Advances in Experimental Medicine and Biology - March 20, 2024 Category: Research Authors: Hassan Hayek Lauriane Gross Fatima Alghoul Franck Martin Gilbert Eriani Christine Allmang Source Type: research