Kinetic modeling and optimization of ethanol fermentation by the marine yeast Wickerhamomyces subpelliculosus ZE75

AbstractThe study aims to enhance ethanol production byWickerhamomyces subpelliculosus ZE75 isolated from marine sediment. In addition, analyzing the kinetic parameters of ethanol production and optimization of the fermentation conditions was performed. The marine yeast isolate ZE75 was selected as the front runner ethanol-producer, with an ethanol yield of 89.77  gL−1. ZE75 was identified relying on the phenotypic and genotypic characteristics ofW. subpelliculosus. The genotypic characterization based on the Internal Transcribed Spacer (ITS) sequence was deposited in the GenBank database with the accession number OP715873. The maximum specific ethanol production rate (vmax) was 0.482  gg−1 h−1 at 175  gL−1 glucose concentration, with a high accuracy ofR2 0.95. The maximum growth specific rates (μmax) were 0.141  h−1 obtained at 150  gL−1 glucose concentration withR2 0.91. Optimization of the fermentation parameters such as pH and salinity has been achieved. The highest ethanol yield 0.5637  gg−1 was achieved in a 100% natural seawater-based medium. The maximum ethanol production of 104.04  gL−1 was achieved at pH 4.5 with a specific ethanol rate of 0.1669  gg−1 h−1. The findings of the present study recommend the possibility of ethanol production from a seawater-based medium on a large scale usingW. subpelliculosus ZE75.Graphical abstract
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research