Measurement of Ca < sup > 2+ < /sup > Uptake Through Connexin Hemichannels

Methods Mol Biol. 2024;2801:97-109. doi: 10.1007/978-1-0716-3842-2_8.ABSTRACTIncreasing evidence points to deregulated flux of ionized calcium (Ca2+) mediated by hyperactive mutant connexin (Cx) hemichannels (HCs) as a common gain-of-function etiopathogenetic mechanism for several diseases, ranging from skin disorders to nervous system defects. Furthermore, the opening of nonmutated Cx HCs is associated with an impressive list of widespread diseases including, but not limited to, ischemia/stroke, Alzheimer's disease, and epilepsy. HC inhibitors are attracting a growing attention due to their therapeutic potential for numerous pathologies. This chapter describes a quantitative method to measure Ca2+ uptake though HCs expressed in cultured cells. The assay we developed can be used to probe HC activity as wells as to test HC inhibitors. Furthermore, with minor changes it can be easily adapted to high-throughput high-content platforms and/or primary cells and microtissues.PMID:38578416 | DOI:10.1007/978-1-0716-3842-2_8
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research