Enrichment methods of N-linked glycopeptides from human serum or plasma: A mini-review

Carbohydr Res. 2024 Mar 15;538:109094. doi: 10.1016/j.carres.2024.109094. Online ahead of print.ABSTRACTHuman diseases often correlate with changes in protein glycosylation, which can be observed in serum or plasma samples. N-glycosylation, the most common form, can provide potential biomarkers for disease prognosis and diagnosis. However, glycoproteins constitute a relatively small proportion of the total proteins in human serum and plasma compared to the non-glycosylated protein albumin, which constitutes the majority. The detection of microheterogeneity and low glycan abundance presents a challenge. Mass spectrometry facilitates glycoproteomics research, yet it faces challenges due to interference from abundant plasma proteins. Therefore, methods have emerged to enrich N-glycans and N-linked glycopeptides using glycan affinity, chemical properties, stationary phase chemical coupling, bioorthogonal techniques, and other alternatives. This review focuses on N-glycans and N-glycopeptides enrichment in human serum or plasma, emphasizing methods and applications. Although not exhaustive, it aims to elucidate principles and showcase the utility and limitations of glycoproteome characterization.PMID:38564900 | DOI:10.1016/j.carres.2024.109094
Source: Carbohydrate Research - Category: Genetics & Stem Cells Authors: Source Type: research