Structure of the K141 capsular polysaccharide produced by Acinetobacter baumannii isolate KZ1106 that carries KL141 at the chromosomal K locus

Carbohydr Res. 2024 Mar 24;538:109097. doi: 10.1016/j.carres.2024.109097. Online ahead of print.ABSTRACTThe structure of the K141 type capsular polysaccharide (CPS) produced by Acinetobacter baumannii KZ1106, a clinical isolate recovered from Kazakhstan in 2016, was established by sugar analyses and one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS was shown to consist of branched tetrasaccharide repeating units (K-units) with the following structure: This structure was found to be consistent with the genetic content of the KL141 CPS biosynthesis gene cluster at the chromosomal K locus in the KZ1106 whole genome sequence. Assignment of the encoded enzymes allowed the first sugar of the K unit to be identified, which revealed that the β-d-GlcpNAc-(1→3)-d-GlcpNAc bond is the linkage between K-units formed by the WzyKL141 polymerase.PMID:38555658 | DOI:10.1016/j.carres.2024.109097

Source: Carbohydrate Research - March 31, 2024 Category: Genetics & Stem Cells Authors: Anastasiya A Kasimova Anna S Kolganova Alexander S Shashkov Mikhail M Shneider Yulia V Mikhailova Andrey A Shelenkov Anastasiya V Popova Yuriy A Knirel Andrey V Perepelov Johanna J Kenyon Source Type: research