Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control

Biochem Genet. 2024 Mar 24. doi: 10.1007/s10528-024-10714-8. Online ahead of print.ABSTRACTBladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. One of its mechanisms of action is the regulation of RECK, a tumor suppressor gene responsible for inhibiting metalloproteinases, including MMP9. In a high-grade urothelial cancer cell line, we aimed to assess if miR-21 downregulation would promote RECK expression and decrease MMP9 expression. We also evaluated cellular migration and proliferation potential by inhibition of this pathway. In a T24 cell line, we inhibited miR-21 expression by transfection of a specific microRNA inhibitor (anti-miR-21). There were also control and scramble groups, the last with a negative microRNA transfected. After the procedure, we performed a genetic expression analysis of miR-21, RECK, and MMP9 through qPCR. Migration, proliferation, and protein expression were evaluated via wound healing assay, colony formation assay, flow cytometry, and immunofluorescence.After anti-miR-21 transfection, miR-21 expression decreased with RECK upregulation and MMP9 downregulation. The immunofluorescence assay showed...

Source: Biochemical Genetics - March 24, 2024 Category: Genetics & Stem Cells Authors: Paulo Rodolfo Moraes Dos Santos Paulo Ricardo da Silva Gomes Poliana Rom ão Feres Camargo Maluf Vanessa Ribeiro Guimar ães Patr ícia Candido Guilherme Lopes Gon çalves Juliana Alves de Camargo Gabriel Arantes Dos Santos Iran Silva Katia Ramos Moreira Source Type: research