Cytogenotoxicity and inflammatory response in liver of rats exposed to different doses of cannabis nano emulsions
Arch Toxicol. 2024 Mar 17. doi: 10.1007/s00204-024-03712-7. Online ahead of print.ABSTRACTCannabis is the most used illicit substance for recreational purposes around the world. However, it has become increasingly common to witness the use of approved cannabis preparations for symptoms management in various diseases. The aim of this study was to investigate the effects of cannabis nano emulsion in the liver of Wistar rats, with different proportions of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). For this, a total of 40 male Wistar rats were distributed into 5 groups, as follows (n = 8 per group): Control: G1, Experimental group (G2): treated with cannabis nano emulsion (THC and CBD) at a dose of 2.5 mg/kg, Experimental group (G3): treated with cannabis nano emulsion (THC and CBD) at a dose of 5 mg/kg, Experimental group (G4): treated with cannabis nano emulsion (CBD) at a dose of 2.5 mg/kg; Experimental group (G5): treated with cannabis nano emulsion (CBD) at a dose of 5 mg/kg. Exposure to the nano emulsion was carried out for 21 days, once a day, orally (gavage). Our results showed that cannabis nano emulsions at higher doses (5 mg/kg), regardless of the composition, induced histopathologic changes in the liver (G3 and G5) in comparison with the control group. In line with that, placental glutathione S-transferase (GST-P) positive foci increased in both G3 and G5 (p < 0.05), as well as the immune expression of Ki-67, vascular endothelial growth factor (VEGF)...
Source: Archives of Toxicology - Category: Toxicology Authors: Thiago Guedes Pinto Barbara Dos Anjos Rosario Andrea Cristina de Moraes Malinverni Rosana Xavier Yasmin Alaby Martins Ferreira Luciana Pellegrini Pisani Pedro Everson Alexandre de Aquino Glauce Socorro de Barros Viana Daniel Vitor de Souza Milena de Barro Source Type: research