Cx < sub > 3 < /sub > Mab-4: A Novel Anti-Mouse CXCR3 Monoclonal Antibody for Flow Cytometry

In this study, we established a novel anti-mouse CXCR3 (mCXCR3) monoclonal antibody, Cx3Mab-4 (rat IgG1, kappa), using the Cell-Based Immunization and Screening method. Flow cytometric analysis demonstrated that Cx3Mab-4 bound to mCXCR3-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR3) cells, but did not react to parental CHO-K1 cells. The dissociation constant of Cx3Mab-4 was determined as 1.3 × 10-9 M, indicating that Cx3Mab-4 possesses a high affinity to mCXCR3-expressing cells. Cx3Mab-4 could be useful for targeting CXCR3-expressing cells in preclinical mouse models.PMID:38507670 | DOI:10.1089/mab.2023.0024
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Authors: Source Type: research