CD32 (Fc γRIIB) expression is low on CD21 < sup > low < /sup > B cells from systemic sclerosis patients with digital ulcers, interstitial lung disease, and anti-topoisomerase I autoantibodies

Clin Immunol. 2024 Mar 15:110195. doi: 10.1016/j.clim.2024.110195. Online ahead of print.ABSTRACTCD21low B cells have recently been found increased in SSc-associated digital ulcers (DUs) or interstitial lung disease (ILD). To further characterize CD21low B cells which encompass autoreactive cells, we analyzed their expression of the inhibitory CD32 receptor in SSc. Peripheral blood mononuclear cells from 27 patients with SSc and 15 age-and sex-matched healthy controls (HCs) were analyzed with multicolor flow cytometry. CD21low B cells were significantly increased in patients with DUs (51.3%) compared to HCs (28.1%) and in patients with ILD (53.1%) compared to HCs. CD21lowCD32low B cells were significantly increased in patients with DUs (23.8%) compared to HCs (4.4%), in patients with ILD (28.4%) compared to HCs, and in anti-topoisomerase I (+) patients (21.5%) compared to HCs and to anti-topoisomerase I (-) patients (2.4%). Autoreactive B cells recognizing Topoisomerase I were predominantly within CD32low cell fraction. Our study further supports the autoreactive status of CD21lowCD32low B cells in SSc patients.PMID:38494058 | DOI:10.1016/j.clim.2024.110195

Source: Clinical Immunology - March 17, 2024 Category: Allergy & Immunology Authors: Evangeli Kourkouni Sotirios G Tsiogkas Athanasios Mavropoulos Theodora Simopoulou Christina G Katsiari Dimitrios P Bogdanos Lazaros I Sakkas Source Type: research