Cell surface β‐lactamase recruitment: A facile selection to identify protein–protein interactions
AbstractProtein –protein interactions (PPIs) are central to many cellular processes, and the identification of novel PPIs is a critical step in the discovery of protein therapeutics. Simple methods to identify naturally existing or laboratory evolved PPIs are therefore valuable research tools. We have developed a facile selection that links PPI-dependent β-lactamase recruitment on the surface ofEscherichia coli with resistance to ampicillin. Bacteria displaying a protein that forms a complex with a specific protein- β-lactamase fusion are protected from ampicillin-dependent cell death. In contrast, bacteria that do not recruit β-lactamase to the cell surface are killed by ampicillin. Given its simplicity and tunability, we anticipate this selection will be a valuable addition to the palette of methods for ill uminating and interrogating PPIs.
Source: Protein Science - Category: Biochemistry Authors: Jordan A. Hinmon,
Jade M. King,
Latrina J. Mayo,
Cierra R. Faries,
Ya'hnis T. Lockett,
David W. Crawford,
Patrick C. Beardslee,
Alexander Hendricks,
Brian R. McNaughton Tags: RESEARCH NOTE Source Type: research
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