Cell surface β‐lactamase recruitment: A facile selection to identify protein–protein interactions

AbstractProtein –protein interactions (PPIs) are central to many cellular processes, and the identification of novel PPIs is a critical step in the discovery of protein therapeutics. Simple methods to identify naturally existing or laboratory evolved PPIs are therefore valuable research tools. We have developed a facile selection that links PPI-dependent β-lactamase recruitment on the surface ofEscherichia coli with resistance to ampicillin. Bacteria displaying a protein that forms a complex with a specific protein- β-lactamase fusion are protected from ampicillin-dependent cell death. In contrast, bacteria that do not recruit β-lactamase to the cell surface are killed by ampicillin. Given its simplicity and tunability, we anticipate this selection will be a valuable addition to the palette of methods for ill uminating and interrogating PPIs.
Source: Protein Science - Category: Biochemistry Authors: Tags: RESEARCH NOTE Source Type: research