Production of non-natural 5-methylorsellinate-derived meroterpenoids in Aspergillus oryzae

In this study, we constructed engineered metabolic pathways in the fungus Aspergillus oryzae to expand the molecular diversity of meroterpenoids. We employed the 5-methylorsellinic acid (5-MOA) synthase FncE and three additional biosynthetic enzymes for the formation of (6R,10′R)-epoxyfarnesyl-5-MOA methyl ester, which served as a non-native substrate for four terpene cyclases from DMOA-derived meroterpenoid pathways. As a result, we successfully generated six unnatural 5-MOA-derived meroterpenoid species, demonstrating the effectiveness of our approach in the generation of structural analogues of meroterpenoids. Beilstein J. Org. Chem. 2024, 20, 638–644. doi:10.3762/bjoc.20.56
Source: Beilstein Journal of Organic Chemistry - Category: Chemistry Authors: Tags: biosynthesis meroterpenoids natural products pathway engineering terpene cyclases Letter Source Type: research