The role of vitamin D receptor in predentin mineralization and dental repair after injury

AbstractDentin is a permeable and complex tubular composite formed by the mineralization of predentin that mineralization and repair are of considerable clinical interest during dentin homeostasis. The role ofVdr, a receptor of vitamin D, in dentin homeostasis remains unexplored. The aim of the present study was to assess the impact ofVdr on predentin mineralization and dental repair.Vdr-knockout (Vdr−/−) mice models were constructed; histology and immunohistochemistry analyses were conducted for bothWT andVdr−/− mice. The finding revealed a thicker predentin inVdr−/− mice, characterized by higher expression of biglycan and decorin. A dental injury model was employed to observe tertiary dentin formation inVdr−/− mice with dental injuries. Results showed that tertiary dentin was harder to form inVdr−/− mice with dental injury. Over time, heightened pulp invasion was observed at the injury site inVdr−/− mice. Expression of biglycan and decorin was reduced in the predentin at the injury site in theVdr−/− mice by immunohistochemistry. Taken together, our results imply thatVdr plays a regulatory role in predentin mineralization and tertiary dentin formation during dentin homeostasis.
Source: Cell and Tissue Research - Category: Cytology Source Type: research