New Dual Inducible Cellular Model to Investigate Temporal Control of Oncogenic Cooperating Genes

bioRxiv [Preprint]. 2024 Feb 27:2024.02.23.581802. doi: 10.1101/2024.02.23.581802.ABSTRACTThe study of cooperating genes in cancer can lead to mechanistic understanding and identifying potential therapeutic targets. To facilitate these types of studies, we developed a new dual-inducible system utilizing the tetracycline- and cumate-inducible systems driving HES3 and the PAX3::FOXO1 fusion-oncogene, respectively, as cooperating genes from fusion-positive rhabdomyosarcoma. With this new model, we can independently induce expression of either HES3 or PAX3::FOXO1, as well as simultaneously induce expression of both genes. This new model will allow us to further investigate the cooperation between HES3 and PAX3::FOXO1 including the temporal requirements for genetic cooperation. This dual-inducible model can be adapted for any cooperating genes, allowing for independent, simultaneous, or temporally controlled gene expression.PMID:38464002 | PMC:PMC10925205 | DOI:10.1101/2024.02.23.581802
Source: Cancer Control - Category: Cancer & Oncology Authors: Source Type: research