Influence of systemic antibiotic therapy on the development and progression of induced apical periodontitis in Wistar rats

AbstractThe aim of this study was to investigate the influence of systemic antibiotic therapy on the development and progression of induced apical periodontitis (AP) in Wistar rats. Fifty-six rats were submitted to pulp exposure of the lower left first molar for the induction of AP. On the same day, intraperitoneal antibiotic therapy was administered once a day, for 15  days, until euthanasia. The groups were formed according to the different treatments (n = 8): C—control; GEN—treated with gentamicin (10 mg/Kg); AC—treated with amoxicillin (100 mg/Kg); MZ—treated with metronidazole (40 mg/Kg); AMP—treated with ampicillin (100 mg/Kg); AMC group—treated with amoxicillin + clavulanic acid (100 mg/kg); CLI—treated with clindamy cin (60 mg/kg). After euthanasia, the jaws were collected and processed for (1) histological and histometric analysis using hematoxylin and eosin staining, (2) analysis of collagen fibers using Picrosirius Red staining and (3) bacteriological analysis using Brown–Brenn staining. The data were ana lyzed statistically (p <  0.05). AP induction was confirmed in all groups. The AMC group had the lower intensity of inflammatory infiltrate (p = 0.028) and less periapical bone resorption compared to control (p = 0.006). Regarding collagen maturation, PSR staining revealed a predominance of mature collagen fibers in all groups. The AC and AMC groups had the lower amount of mature fibers and the highest amount of immatu...
Source: Odontology - Category: Dentistry Source Type: research