Role of CYP311A1 in wing development of Drosophila melanogaster

In this study, we investigated the role of CYP311A1 in wing morphogenesis inDrosophila. Using the GAL4-UAS system,Cyp311a1 was selectively knocked down in the wing disc. A deformed wing phenotype was observed in flies with reducedCyp311a1 transcripts. BODIPY and oil red O staining revealed a reduction of neutral lipids in the wing disc after the depletion ofCyp311a1. In addition, we observed an enhanced sensitivity to Eosin Y penetration in the wings ofCyp311a1 knocked-down flies. Moreover, the reduction of CYP311A1 function in developing wings does not affect cell proliferation and apoptosis, but entails disordered Phalloidin or Cadherin distribution, suggesting an abnormal cell morphology and cell cortex structure in wing epithelial cells. Taken together, our results suggest thatCyp311a1 is needed for wing morphogenesis by participating in lipid assembly and cell homeostasis.

Source: Insect Science - March 6, 2024 Category: Biology Authors: Xubo Zhang, Mengqi Liu, Andi Cheng, Bernard Moussian, Jianzhen Zhang, Wei Dong Tags: ORIGINAL ARTICLE Source Type: research