Fine mapping of a major QTL, qKl-1BL controlling kernel length in common wheat
In this study, a major QTL for kernel length (KL) on 1BL, named qKl-1BL, was identified from the recombinant inbred lines (RIL) in multiple environments based on the genetic map and physical map, with 4.76-21.15% of the phenotypic variation explained. To fine map qKl-1BL, the map-based cloning strategy was used. By using developed InDel markers, the near-isogenic line (NIL) pairs and eight key recombinants were identified from a segregating population containing 3621 individuals derived from residual heterozygous lines (RHLs) self-crossing. In combination with phenotype identification, qKl-1BL was finely positioned into a 2.04-Mb interval, KN1B:698.15-700.19 Mb, with eight differentially expressed genes enriched at the key period of kernel elongation. Based on transcriptome analysis and functional annotation information, two candidate genes for qKl-1BL controlling kernel elongation were identified. Additionally, genetic effect analysis showed that the superior allele of qKl-1BL from Jing411 could increase KL, thousand kernel weight (TKW), and yield per plant (YPP) significantly, as well as kernel bulk density and stability time. Taken together, this study identified a QTL interval for controlling kernel length with two possible candidate genes, which provides an important basis for qKl-1BL cloning, functional analysis, and application in molecular breeding programs.PMID:38441674 | DOI:10.1007/s00122-024-04574-4
Source: TAG. Theoretical and Applied Genetics - Category: Genetics & Stem Cells Authors: Ran Qin Mingsu Cao Jizi Dong Linqu Chen Haoru Guo Qingjie Guo Yibiao Cai Lei Han Zhenjie Huang Ninghao Xu Aoyu Yang Huiyuan Xu Yongzhen Wu Han Sun Xigang Liu Hongqing Ling Chunhua Zhao Junming Li Fa Cui Source Type: research