Determining individual glomerular proteinuria and periglomerular infiltration in a cleared murine kidney by a 3D fast-marching algorithm.

Three-dimensional imaging has advanced basic research and clinical medicine. However, limited resolution and imperfections of real-world 3D image material often preclude algorithmic image analysis. Here, we present a methodological framework for such imaging and analysis for functional and spatial relations in experimental nephritis. First, optical tissue clearing protocols were optimized to preserve fluorescence signals for light-sheet-fluorescence-microscopy and compensated attenuation effects using adjustable 3D correction fields.
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: technical notes Source Type: research