Lipopolysaccharide-induced intestinal inflammation on AIM2-mediated pyroptosis in the brain of rats with cerebral small vessel disease

This study investigated whether lipopolysaccharide-induced intestinal inflammation enhanced the level of pyroptosis in the brain of rats with CSVD. The bilateral carotid artery occlusion (BCAO) model was selected as the object of study. Firstly, behavioral tests and Hematoxylin-eosin staining (HE staining) were performed to determine whether the model was successful, and then AIM2-mediated inflammasome and pyroptosis indexes(GSDMD、N-GSDMD)in brain tissue were detected by Western blotting and Immunohistochemistry. Finally, a single intraperitoneal injection of lipopolysaccharide (LPS) was used to induce intestinal inflammation in rats, the expression level of GSDMD and N-GSDMD in the brain was analyzed by Western blotting and to see if pyroptosis caused by intestinal inflammation can be inhibited by Disulfiram, an inhibitor of pyroptosis. The results showed that the inflammatory response and pyroptosis mediated by AIM2 inflammasomes in BCAO rats were present in both brain and intestinal tissues. The expression of N-GSDMD, a key marker of pyroptosis, in brain tissue was significantly increased and inhibited by Disulfiram after LPS-induced enhancement of intestinal inflammation. This study shows that AIM2-mediated inflammasome activation and pyroptosis exist in both brain and intestinal tissues in the rat model of CSVD. The enhancement of intestinal inflammation will increase the level of pyroptosis in the brain. In the future, targeted regulation of AIM2 inflammasome activati...

Source: Experimental Neurology - March 1, 2024 Category: Neurology Authors: Huiping Xu Han Xiao Qiqiang Tang Source Type: research