< em > RASGRP1 < /em > targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs

In this study, we verify the crucial role of H3K27me3 in RASGRP1 regulation. The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells (PSCs). The results of qPCR, western blot analysis, EdU staining, CCK-8 assay and immunofluorescence staining show that overexpression of RASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models, while knockdown of RASGRP1 leads to the opposite results. These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mice and pigs.PMID:38419500 | DOI:10.3724/abbs.2024011
Source: Acta Biochimica et Biophysica Sinica - Category: Biochemistry Authors: Source Type: research