Genes, Vol. 15, Pages 305: Developmental Changes in Genome Replication Progression in Pluripotent versus Differentiated Human Cells

Genes, Vol. 15, Pages 305: Developmental Changes in Genome Replication Progression in Pluripotent versus Differentiated Human Cells Genes doi: 10.3390/genes15030305 Authors: Sunil Kumar Pradhan Teresa Lozoya Paulina Prorok Yue Yuan Anne Lehmkuhl Peng Zhang M. Cristina Cardoso DNA replication is a fundamental process ensuring the maintenance of the genome each time cells divide. This is particularly relevant early in development when cells divide profusely, later giving rise to entire organs. Here, we analyze and compare the genome replication progression in human embryonic stem cells, induced pluripotent stem cells, and differentiated cells. Using single-cell microscopic approaches, we map the spatio-temporal genome replication as a function of chromatin marks/compaction level. Furthermore, we mapped the replication timing of subchromosomal tandem repeat regions and interspersed repeat sequence elements. Albeit the majority of these genomic repeats did not change their replication timing from pluripotent to differentiated cells, we found developmental changes in the replication timing of rDNA repeats. Comparing single-cell super-resolution microscopic data with data from genome-wide sequencing approaches showed comparable numbers of replicons and large overlap in origins numbers and genomic location among developmental states with a generally higher origin variability in pluripotent cells. Using ratiometric analysis of incorporated nucleotides normalized p...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research