Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo
This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT) in Drosophila melanogaster, and the anti-angiogenic effect by the chick embryo chorioallantoic membrane (CAM) assay. Data demonstrated that DvL promoted strong selective cytotoxicity against tumor cell lines, especially A549 and S180 cells, whereas normal cell lines were weakly affected. Furthermore, DvL did not promote carcinogenesis in D. melanogaster at any concentration tested, but modulated DXR-induced carcinogenesis at the highest concentrations tested. In the CAM and immunohistochemical assays, DvL inhibited sarcoma 180-induced angiogenesis and promoted the reduction of VEGF and TGF-β levels at all concentrations tested. Therefore, our results demonstrated that DvL is a potent anticancer, anti-angiogenic, and selective cytotoxic agent for tumor cells, suggesting its potential application as a prototype molecule for the development of new drugs with chemoprotective and/or antitumor effects.PMID:38395337 | DOI:10.1016/j.biochi.2024.02.007
Source: Biochimie - Category: Biochemistry Authors: Abel Vieira de Melo Bisneto Amanda Silva Fernandes L ívia do Carmo Silva Luana Santos Silva Diego Pereira de Ara újo Ivan Cerqueira Dos Santos Marcella da Rocha Melo Rom ério Rodrigues Dos Santos Silva Leonardo Pereira Franchi Clever Gomes Cardoso Elis Source Type: research