Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation

Cryobiology. 2024 Feb 20:104867. doi: 10.1016/j.cryobiol.2024.104867. Online ahead of print.ABSTRACTPollen, as the male gametophyte, carries half of plant genetic information and is an important source of germplasm. The cryopreservation of pollen can not only preserve germplasm, but also solve the problem of time and space barrier in crossbreeding. So it is of great significance to explore the mechanism of pollen viability maintenance after cryopreservation. In this paper, 10 cultivars of Paeonia lactiflora with different fresh pollen viability that did not change after cryopreservation were taken as objects and the effects of pollen inclusions such as soluble sugars, starch, soluble proteins, free amino acids, and proline were explored. The results showed that: 1) The contents of pollen inclusions in the fresh pollen of 10 cultivars were different. After cryopreservation, the contents of starch and free amino acids significantly decreased in 10 cultivars, and the soluble sugar, soluble protein, and proline varied with cultivars. 2) Correlation analysis showed that fresh pollen viability was significantly positively correlated with the soluble sugar (R-values of 0.630) and starch content (R-values of 0.694) in fresh pollen. But after cryopreservation pollen viability was only significantly positively correlated with the starch content (R-values of 0.725). These results suggest that the effects of pollen inclusions on pollen vitality are different before and after cryopreserva...

Source: Cryobiology - February 22, 2024 Category: Biology Authors: Mengting Zhu Ying Peng Jianzhou Gao Ruifen Ren Yingling Wan Yan Liu Source Type: research