Strigolactone biosynthesis in rice can occur via a 9 ‐cis‐3‐OH‐10′‐apo‐β‐carotenal intermediate

In this study, we supplied the13C-labeled 9-cis-3-OH- β-apo-10′-carotenal to rice seedlings and monitored the synthesis of SLs using liquid chromatography-mass spectrometry (LC–MS) andStriga bioassay. We further validated the biological activity of 9-cis-3-OH- β-apo-10′-carotenal-derived SLs using theccd7/d17 SL-deficient mutant, which demonstrated increasedStriga seed-germinating activity and partial rescue of tiller numbers and plant height. Our results establish 9-cis-3-OH- β-apo-10′-carotenal as a significant SL biosynthetic intermediate with implications for understanding plant hormonal functions and potential applications in agriculture.

Source: FEBS Letters - February 20, 2024 Category: Biochemistry Authors: Jian You Wang, Guan ‐Ting Erica Chen, Aparna Balakrishna, Muhammad Jamil, Lamis Berqdar, Salim Al‐Babili Tags: Research Letter Source Type: research