Ameliorative effects of Atriplex crassifolia (C.A.Mey) on pain and inflammation through modulation of inflammatory biomarkers and GC –MS-based metabolite profiling

AbstractAtriplex crassifolia (A. crassifolia) is a locally occurring member of Chenopodiaceae family that has been used in folk medicine for the treatment of joint pain and inflammation. The present study was focused to determine the analgesic and anti-inflammatory potential of the plant. n-hexane (ACNH) and methanol (ACM) extracts ofA. crassifolia were evaluated for in vitro anti-inflammatory potential using protein denaturation inhibition assay. In vivo anti-inflammatory potential was determined by oral administration of 250, 500, and 1000  mg/kg/day of extracts against carrageenan and formalin-induced paw edema models. Inflammatory mediators such as TNF-α, IL-10, IL-1β, NF-kB, IL-4, and IL-6 were estimated in blood samples of animals subjected to formalin model of inflammation. Analgesic activity was determined using acetic acid-i nduced writhing and tail flick assay model. Phytochemical profiling was done by GC–mass spectrophotometer. The results of in vitro anti-inflammatory activity revealed that both ACNH and ACM displayed eminent inhibition of protein denaturation in concentration-dependent manner. In acute in vivo car rageenan-induced paw edema model, both extracts reduced inflammation at 5th and 6th hour of study (p <  0.05).A. crassifolia extracts exhibited significant inhibition against formalin-induced inflammation with maximum effect at 1000  mg/kg. ACNH and ACM significantly augmented the inflammatory mediators (p <  0.05). Levels of TNF-α,...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research