Stability of anti-drug antibodies in human serum samples

J Immunol Methods. 2024 Feb;525:113616. doi: 10.1016/j.jim.2024.113616. Epub 2024 Jan 10.ABSTRACTStability of anti-drug antibodies (ADAs) is important as ADA-analysis should be reliable over time at different storage conditions. Stability of anti-insulin antibodies in serum samples was assessed after short-term storage at different temperatures and after long-term storage at -20 °C. Correlation between measurements was tested and acceptance criteria for incurred sample reanalysis were applied. ADAs were stable after 72 h at 22 °C, after 2 weeks at 4 °C, and after 6.3 years at -20 °C. The study confirms that ADAs in serum are stable for several years at -20 °C and suggests that investigation of short- and long-term stability of ADAs is not needed if samples are handled at standard laboratory-conditions.PMID:38211695 | DOI:10.1016/j.jim.2024.113616
Source: Journal of Immunological Methods - Category: Allergy & Immunology Authors: Source Type: research