In adult X-CGD patients, regulatory T cells are expanded while activated T cells display a NOX2-independent ROS increase
Immunol Lett. 2024 Feb 1;266:106839. doi: 10.1016/j.imlet.2024.106839. Online ahead of print.ABSTRACTThe X-linked chronic granulomatous disease (X-CGD), a rare genetic disease characterised by recurrent infections, is caused by mutations of NOX2. Significant proportions of X-CGD patients display signs of immune dysregulation. Regulatory T cells (Tregs) are CD4+T lymphocytes that expand in active inflammation and prevent autoimmune disorders. Here we asked whether X-CGD is associated to Treg dysfunctions in adult patients. To this aim, the frequency of Tregs was analysed through intracellular flow cytometry in a cohort of adult X-CGD patients, carriers and controls. We found that Tregs were significantly expanded and activated in blood of adult X-CGD patients, and this was associated with activation of conventional CD4+T cells (Tconvs). T cell activation was characterised by accumulation of intracellular ROS, not derived from NOX2 but likely produced by cellular metabolism. The higher TNF production by Tconvs in X-CGD patients might contribute to the expansion of Tregs through the TNFR2 receptor. In summary, our data indicate that Tregs expand in adult X-CGD in response to immune activation, and that the increase of NOX2-independent ROS content is a feature of activated T cells.PMID:38309375 | DOI:10.1016/j.imlet.2024.106839
Source: Immunology Letters - Category: Allergy & Immunology Authors: Ilenia Cammarata Valeria Pinna Ilenia Pacella Ivano Rotella Annarosa Soresina Raffaele Badolato Alessandro Plebani Claudio Pignata Emilia Cirillo Anna Maria Zicari Francesco Violi Roberto Carnevale Lorenzo Loffredo Silvia Piconese Source Type: research