PCGF6 controls murine Tuft cell differentiation via H3K9me2 modification independently of Polycomb repression

We report that PCGF6 regulates mouse intestinal Tuft cell differentiation independently of H2AK119ub1 deposition. We show that PCGF6 chromatin occupancy expands outside Polycomb repressive domains, associating with unique promoter and distal regulatory elements. This occurs in the absence of RING1A/B and involves MGA-mediated E-BOX recognition and specific H3K9me2 promoter deposition. PCGF6 inactivation induces an epithelial autonomous accumulation of Tuft cells that was not phenocopied by RING1A/B loss. This involves direct PCGF6 association with a Tuft cell differentiation program that identified Polycomb-independent properties of PCGF6 in adult tissues homeostasis.PMID:38228142 | DOI:10.1016/j.devcel.2023.12.015

Source: Developmental Cell - January 16, 2024 Category: Cytology Authors: Annachiara Del Vecchio Patrizia Mul é Daniel Fern ández-Pérez Simona Amato Georgia Lattanzi Marika Zanotti Samantha Rustichelli Silvia Pivetti Paola Oldani Andrea Mariani Fabiola Iommazzo Haruhiko Koseki Federica Facciotti Simone Tamburri Karin J Ferra Source Type: research