Effect of chemical and physical agents on monkeypox virus infectivity and downstream research applications

Virology. 2024 Jan 16;592:109993. doi: 10.1016/j.virol.2024.109993. Online ahead of print.ABSTRACTThe 2022 global spread of Monkeypox Virus (MPXV) underlined the need to investigate safe-handling procedures of clinical and research samples. Here we evaluated the efficiency in reducing MPXV infectious titer of Triton X-100 (0.1 and 0.2%), UV-C irradiation (15 or 30 min), and heat (56 °C 30 min or 70 °C 5 min). The treatment of MPXV at 70 °C resulted in the strongest decrease of MPXV infectious titer (5.4 Log TCID50/mL), 56 °C and UV-C had a lighter impact (3.9 and 4.3Log), Triton X-100 was less efficient (1.8-2.5Log). Notably, SARS-CoV-2 was much more susceptible to Triton X-100 (4.0 Log decrease). UV-C had the highest impact on MPXV DNA detection by PCR (2.2-4.3 Ct value increase); protein detection by ELISA was dramatically impaired by heating. Overall, UV-C and heating were more effective in lowering MPXV infectious titer but their impact on nucleic acids or protein detection assays must be considered.PMID:38244323 | DOI:10.1016/j.virol.2024.109993

Source: Virology - January 20, 2024 Category: Virology Authors: Davide Mariotti Aurora Bettini Silvia Meschi Stefania Notari Massimo Francalancia Eleonora Tartaglia Daniele Lapa Eliana Specchiarello Enrico Girardi Giulia Matusali Fabrizio Maggi Source Type: research