Development of a Sensitive Anti-Mouse CD39 Monoclonal Antibody (C < sub > 39 < /sub > Mab-1) for Flow Cytometry and Western Blot Analyses

This study developed specific and sensitive mAbs for mouse CD39 (mCD39) using the Cell-Based Immunization and Screening method. The established anti-mCD39 mAb, C39Mab-1 (rat IgG2a, kappa), reacted with mCD39-overexpressed Chinese hamster ovary-K1 (CHO/mCD39) by flow cytometry. The kinetic analysis using flow cytometry indicated that the dissociation constant of C39Mab-1 for CHO/mCD39 was 7.3 × 10-9 M. Furthermore, C39Mab-1 detected the lysate of CHO/mCD39 by western blot analysis. These results indicated that C39Mab-1 is useful for the detection of mCD39 in many functional studies.PMID:38197855 | DOI:10.1089/mab.2023.0016
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Authors: Source Type: research