Excited state properties of 5 ‐fluoro‐4‐thiouridine derivative†

The excited state properties of 2 ′,3′,5′-tri-O-acetyl-5-fluoro-4-thiouridine in acetonitrile have been intensively investigated. The intrinsic triplet lifetime and the formation quantum yields of the excited triplet state and the singlet molecular oxygen were successfully determined. The fraction of triplet ta5F4TUrd quenched by dissolved oxygen which gives rise to the1O2* formation was the largest among the thionucleobases and the thionucleosides reported so far. AbstractThe excited state properties of thionated 5-fluorouridine (2 ′,3′,5′-tri-O-acetyl-5-fluoro-4-thiouridine; ta5F4TUrd), synthesized with Lawesson's reagent, have been intensively investigated with nanosecond transient absorption spectroscopy, time-resolved thermal lensing, near-infrared emission, and quantum chemical calculation. The intrinsic triplet lifetime of ta5F4TUrd was determined to be 4.2 ±0.7$$ 4.2\pm 0.7 $$ μs in acetonitrile, and the formation quantum yield of the excited triplet state was as large as 0.79±0.01$$ 0.79\pm 0.01 $$. The quenching rate constants of the triplet ta5F4TUrd by the dissolved oxygen molecule and by the self-quenching process were found to be nearly equal to the diffusion-controlled rate of acetonitrile. The quantum yield of the singlet molecular oxygen produced through energy transfer between the triplet ta5F4TUrd and the dissolved oxygen, ΦΔ$$ {\varPhi}_{\Delta} $$, was successfully determined to be 0.61±0.02$$ 0.61\pm 0.02 $$ under the oxygen- saturated ...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: SPECIAL ISSUE RESEARCH ARTICLE Source Type: research