Viruses, Vol. 16, Pages 170: Molecular Amplification and Cell Culturing Efficiency for Enteroviruses & rsquo; Detection in Cerebrospinal Fluids of Algerian Patients Suffering from Meningitis
Viruses, Vol. 16, Pages 170: Molecular Amplification and Cell Culturing Efficiency for Enteroviruses’ Detection in Cerebrospinal Fluids of Algerian Patients Suffering from Meningitis
Viruses doi: 10.3390/v16020170
Authors:
Abdelwahab Rai
Zohra Ammi
Dahbia Leila Anes-Boulahbal
Aymen Amin Assadi
Abdeltif Amrane
Oussama Baaloudj
Lotfi Mouni
Enteroviruses (EVs) represent a major cause of viral meningitis, being responsible for nearly 1 billion infections each year worldwide. Several techniques were developed to obtain better diagnostic results of EV infections. Herein, we evaluated the efficiency of EV detection through isolation on both Rhabdomyosarcoma (RD) and Vero cell line cultures, conventional reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR. Thus, 50 cerebrospinal fluid (CSF) samples belonging to patients suspected to have viral meningitis in northern Algeria were collected, anonymously numbered from 1 to 50 and subjected to the above-mentioned techniques for EV detection. Using real-time RT-PCR, 34 CSF samples were revealed to be positive for viral origin of meningitis (68%). Thirteen of them were positive when the conventional RT-PCR was used (26%), and only three samples gave positive results when the cell culture technique was used (6%). Surprisingly, two cell culture-positive CSF samples, namely, 31 and 39, were negative using RT-PCR directly on the original samples. However, they turned to be positive when a...
Source: Viruses - Category: Virology Authors: Abdelwahab Rai Zohra Ammi Dahbia Leila Anes-Boulahbal Aymen Amin Assadi Abdeltif Amrane Oussama Baaloudj Lotfi Mouni Tags: Article Source Type: research