Genes, Vol. 15, Pages 97: AcMYB10 Involved in Anthocyanin Regulation of & lsquo;Hongyang & rsquo; Kiwifruit Induced via Fruit Bagging and High-Postharvest-Temperature Treatments

Genes, Vol. 15, Pages 97: AcMYB10 Involved in Anthocyanin Regulation of ‘Hongyang’ Kiwifruit Induced via Fruit Bagging and High-Postharvest-Temperature Treatments Genes doi: 10.3390/genes15010097 Authors: Min Yu Jinyu Xiong Kun Dong Xin Quan Hao Guo Junwei Huo Dong Qin Yanchang Wang Xuemei Lu Chenqiao Zhu Light and temperature are key factors influencing the accumulation of anthocyanin in fruit crops. To assess the effects of fruit bagging during development and high post-ripening temperature on ‘Hongyang’ kiwifruit, we compared the pigmentation phenotypes and expression levels of anthocyanin-related genes between bagged and unbagged treatments, and between 25 °C and 37 °C postharvest storage temperatures. Both the bagging and 25 °C treatments showed better pigmentation phenotypes with higher anthocyanin concentrations. The results of the qRT-PCR analysis revealed that the gene expression levels of LDOX (leucoanthocyanidin dioxygenase), F3GT (UDP-flavonoid 3-O-glycosyltransferase ), AcMYB10, and AcbHLH42 were strongly correlated and upregulated by both the bagging treatment and 25 °C storage. The results of bimolecular fluorescence complementation and luciferase complementation imaging assays indicated an interaction between AcMYB10 and AcbHLH42 in plant cells, whereas the results of a yeast one-hybrid assay further demonstrated that AcMYB10 activated the ...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
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