Versatile and automated workflow for the analysis of oligodendroglial calcium signals

We describe a new analytical pipeline containing two free, open source and cross-platform software programs, Occam and post-prOccam, that enable the fully automated analysis of one- and two-photon Ca2+ imaging datasets from oligodendroglia obtained by either ex vivo or in vivo Ca2+ imaging techniques. Easily configurable, our software solution is optimized to obtain unbiased results from large datasets acquired with different imaging techniques. Compared to other recent software, our solution proved to be fast, low memory demanding and faithful in the analysis of oligodendroglial Ca2+ signals in all tested imaging conditions. Our versatile and accessible Ca2+ imaging data analysis tool will facilitate the elucidation of Ca2+-mediated mechanisms in oligodendroglia. Its configurability should also ensure its suitability with new use cases such as other glial cell types or even cells outside the CNS.PMID:38194116 | DOI:10.1007/s00018-023-05065-3

Source: Cellular and Molecular Life Sciences : CMLS - January 9, 2024 Category: Cytology Authors: Dorien A Maas Blandine Manot-Saillet Philippe Bun Chlo é Habermacher Corinne Poilbout Filippo Rusconi Maria Cecilia Angulo Source Type: research