Evolution and separation of actinobacterial pyranose and < em > C < /em > -glycoside-3-oxidases

Appl Environ Microbiol. 2024 Jan 5:e0167623. doi: 10.1128/aem.01676-23. Online ahead of print.ABSTRACTC-Glycosides often form active compounds in various plants. Breakage of the C-C bond in these glycosides to release the aglycone is challenging and proceeds via a two-step reaction, the oxidation of the sugar and subsequent cleavage of the C-C bond. Recently, an enzyme from a soil bacterium, FAD-dependent C-glycoside-3-oxidase (CGOx), was shown to catalyze the initial oxidation reaction. Here, we show that CGOx belongs to the same sequence space as pyranose oxidase (POx), and that an actinobacterial ancestor of the POx/CGOx family evolved into four clades, two of which show a high preference for C-glycosides.PMID:38179968 | DOI:10.1128/aem.01676-23
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research