PDAT1 genome editing reduces hydroxy fatty acid production in transgenic Arabidopsis

In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol.PMID:38053289

Source: BMB Reports - December 6, 2023 Category: Biochemistry Authors: Mid-Eum Park Hyun Uk Kim Source Type: research