Production of cello-oligosaccharides from corncob residue by degradation-synthesis reactions

In this study, two strategies were compared for their ability to transform CCR into cello-oligosaccharides (COS). The first strategy employed the use of endo-glucanases. Although selected endo-glucanases from GH9, GH12, GH45, and GH131 could release COS with degrees of polymerization from 2 to 4, the degrading efficiency was low. For the second strategy, first, CCR was efficiently depolymerized to glucose and cellobiose using the cellulase fromTrichoderma reesei. Then, using these simple sugars and sucrose as the starting materials, phosphorylases from different microorganisms were combined to generate COS to a level up to 100.3  g/L with different patterns and degrees of polymerization. Using tomato as a model plant, the representative COS obtained fromBaSP (a sucrose phosphorylase fromBifidobacterium adolescens),CuCbP (a cellobiose phosphorylase fromCellulomonas uda), andCcCdP (a cellodextrin phosphorylase fromClostridium cellulosi) were shown to be able to promote plant growth. The current study pointed to an approach to make use of CCR for production of the value-added COS.Key points•Sequential use of cellulase and phosphorylases effectively generated cello-oligosaccharides from corncob residue.•Cello-oligosaccharides patterns varied in accordance to cellobiose/cellodextrin phosphorylases.•Spraying cello-oligosaccharides promoted tomato growth.Graphical Abstract

Source: European Journal of Applied Physiology - January 5, 2024 Category: Physiology Source Type: research