Construction of rBCG carrying the IL-2-BZLF1 fusion gene and its immunological function

AbstractIn this research, a recombinant Bacillus Calmette Guerin (rBCG) vector vaccine carrying a humanIL-2 and EBVBZLF1 fusion gene (IL-2-BZLF1-rBCG) was constructed. TheIL-2-BZLF1-rBCG construct was successfully generated and stably expressed the IL-2 and BZLF1 proteins.IL-2-BZLF1-rBCG activated the immune system and promoted the secretion of IFN- γ and TNF-α by CD4+ and CD8+ T cells.IL-2-BZLF1-rBCG activated lymphocytes to effectively kill EBV-positive NPC cells in vitro. Additionally,IL-2-BZLF1-rBCG stimulated the proliferation of NK cells and lymphocytes in vivo, activated related immune responses, and effectively treated EBV-positive NPC. The immune response to and pharmacological effect of IL-2-BZLF1-rBCG were explored in vitro and in vivo to provide a theoretical and experimental basis for the prevention and treatment of EBV-positive tumors with an rBCG vector vaccine.Key points•rBCG with human IL-2 and BZLF1 of EB virus was constructed•The IL-2-BZLF1 fusion gene was stably expressed with rBCG•rBCG with IL-2-BZLF1 has an obvious immune response in vitro and in vivo
Source: European Journal of Applied Physiology - Category: Physiology Source Type: research