Chitosan-based matrix as a carrier for bacteriophages

This study is focused on the preparation and characteristics of chitosan-based material in the form of a film with the addition ofPseudomonas lytic phages (KTN4, KT28, and LUZ19), which would exhibit antibacterial activity as a potential dressing that accelerates the wound healing. We investigated the method of producing a polymer based on microcrystalline chitosan (MKCh) to serve as the matrix for phage deposition. We described some important parameters such as average molar mass, swelling capacity, surface morphology, phage release profile, and antibacterial activity tested in thePseudomonas aeruginosa bacterial model. The chitosan polysaccharide turned out to interact with phage particles immobilizing them within a material matrix. Nevertheless, with the high hydrophilicity and swelling features of the prepared material, the external solution of bacterial culture was absorbed and phages went in direct contact with bacteria causing their lysis in the polymer matrix.Key points• A novel chitosan-based matrix with the addition of active phages was prepared• Phage interactions with the chitosan matrix were determined as electrostatic• Phages in the matrix work through direct contact with the bacterial cells

Source: European Journal of Applied Physiology - January 2, 2024 Category: Physiology Source Type: research