Priming Chondrocytes During Expansion Alters Cell Behavior and Improves Matrix Production in 3D Culture

Cartilage tissue engineering strategies that use autologous chondrocytes require in vitro expansion of cells to obtain enough cells to produce functional engineered tissue. However, chondrocytes dedifferentiate during expansion culture, limiting their ability to produce chondrogenic tissue and their utility for cell-based cartilage repair strategies. The current study identified conditions that favor cartilage production and the mechanobiological mechanisms responsible for these benefits.

Source: Osteoarthritis and Cartilage - December 29, 2023 Category: Rheumatology Authors: Emily D. Lindberg, Tiffany Wu, Kristen L. Cotner, Amanda Glazer, Amir A. Jamali, Lydia L. Sohn, Tamara Alliston, Grace D. O ’Connell Source Type: research