Adiponectin affects ileal contractility of mouse preparations

Am J Physiol Gastrointest Liver Physiol. 2023 Dec 19. doi: 10.1152/ajpgi.00203.2023. Online ahead of print.ABSTRACTAdiponectin (ADPN) has been reported to induce inhibitory effects on gastric motor activity which, being a source of peripheral satiety signals, would contribute to the central anorexigenic effects of the hormone in rodents. However, peripheral satiety signals can also originate from the small intestine. Since there are no data on the effects of ADPN in this gut region, the present study aimed to investigate whether ADPN affects murine ileal contractility. Immunofluorescence experiments and Western Blot were also performed to reveal the expression of ADPN receptors. Mechanical responses of ileal preparations were recorded in vitro via force-displacement transducers. Preparations showed a tetrodotoxin- and atropine-insensitive spontaneous contractile activity. Electrical field stimulation (EFS) induced tetrodotoxin- and atropine-sensitive contractile responses. ADPN induced a decay of the basal tension and decreased the amplitude of either the spontaneous contractility or the EFS-induced excitatory responses. All ADPN effects were abolished by the nitric oxide (NO) synthesis inhibitor, L-NNA. The expression of the ADPN receptor AdipoR1, but not AdipoR2, was also revealed on enteric glial cells. The present results offer the first evidence that ADPN acts on ileal preparations. The hormone exerts inhibitory effects likely involving AdipoR1 on enteric glial cells and...
Source: Am J Physiol Gastroi... - Category: Gastroenterology Authors: Source Type: research