Cx < sub > 4 < /sub > Mab-1: A Novel Anti-Mouse CXCR4 Monoclonal Antibody for Flow Cytometry

This study established a novel anti-mouse CXCR4 (mCXCR4) mAb using the Cell-Based Immunization and Screening method. Flow cytometric analysis showed that an anti-mCXCR4 mAb, Cx4Mab-1 (rat IgG2a, kappa), recognized mCXCR4-overexpressed Chinese hamster ovary-K1 (CHO/mCXCR4) cells and endogenously mCXCR4-expressing mouse myeloma P3X63Ag8U.1 (P3U1) cells. Furthermore, Cx4Mab-1 did not recognize mCXCR4-knockout P3U1 cells. The dissociation constants of Cx4Mab-1 for CHO/mCXCR4 and P3U1 were determined as 6.4 × 10-9 M and 2.3 × 10-9 M, respectively, indicating that Cx4Mab-1 possesses a high affinity to both endogenous and exogenous mCXCR4-expressing cells. These results indicate that Cx4Mab-1 could be a useful tool for preclinical mouse models.PMID:38126879 | DOI:10.1089/mab.2023.0023

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - December 21, 2023 Category: Microbiology Authors: Tsunenori Ouchida Hiroyuki Suzuki Tomohiro Tanaka Mika K Kaneko Yukinari Kato Source Type: research