The presence of Cystic Fibrosis Related Diabetes modifies the sputum microbiome in Cystic Fibrosis disease

Am J Physiol Lung Cell Mol Physiol. 2023 Dec 12. doi: 10.1152/ajplung.00219.2023. Online ahead of print.ABSTRACTCystic fibrosis related diabetes (CFRD) affects 40-50% of adults with CF and is associated with a decline in respiratory health. The microbial flora of the lung is known to change with the development of CF disease, but how CFRD affects the microbiome has not been described. We analysed the microbiome in sputa from 14 people with CF and 14 with CFRD and two who were classed as pre-CFRD by extracting DNA and amplifying the variable V3-V4 region of the microbial 16S gene by PCR. Sequences were analysed and source identified to genus level. We found that the a-diversity of the microbiome using Shannon's diversity index was increased in CFRD compared to CF. Bray Curtis dissimilarity analysis showed that there was separation of the microbiomes in CF and CFRD sputa. The most abundant phyla identified in the sputum samples were Firmicutes and Proteobacteria, Actinobacteriota and Bacteroidota and the ratio of Firmicutes/Bacteroidota was reduced in CFRD compared to CF. Pseudomonas, Azhorizophilus, Porphyromonas and Actinobacillus were more abundant in CFRD compared to CF whereas Staphylococcus was less abundant. The relative abundance of these genera did not correlate with age, some correlated with a decline in FEV1/FVC but all correlated with HbA1c indicating that development of CFRD mediates further changes to the respiratory microbiome in CF.PMID:38084404 | DOI:10.1152/aj...
Source: Am J Physiol Lung Ce... - Category: Respiratory Medicine Authors: Source Type: research