An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures

AbstractFASTinov ® developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov® sampleprep was tested for identification (ID) by matrix absorption laser deionization –time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep  was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9% for Gram-positive and 96.3% for Gram-negative bacteria.
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research