Profiling human brain vascular cells using single-cell transcriptomics and organoids

Nature Protocols, Published online: 15 December 2023; doi:10.1038/s41596-023-00929-1A protocol extension describing the purification of prenatal human brain endothelial and mural cells with FACS and their utilization in downstream applications, including cell culture, organoid transplantation and single-cell transcriptomics.

Source: Nature Protocols - December 15, 2023 Category: Biomedical Science Authors: Elizabeth E. Crouch Loukas N. Diafos Edward J. Valenzuela Kaylee Wedderburn-Pugh Janeth Ochoa Birrueta Jaela Caston Tara Joseph Madeline G. Andrews Aparna Bhaduri Eric J. Huang Source Type: research