Prepared Plasmonic Glass Substrates via Electrodeposition for Detecting Trace Glucose: SERS, DFT, and FDTD Investigations

AbstractGlucose plays a key role in the regulation of the levels of blood sugar, and the body closely monitors it to maintain proper metabolic function. Although glucose is essential for human health, its excessive consumption can lead to negative effects such as obesity and diabetes. As a result, high-sensitive and accurate glucose detection is considered an essential measure in clinical analysis. Surface-enhanced Raman scattering (SERS) is an accurate method for identifying trace substances. In the present study, SERS-active substrates were used for detecting different concentrations of glucose. First, gold nanoparticles (AuNPs) were prepared by employing a chemical reduction method. Afterward, the fluorine-doped tin oxide (FTO) glass substrates were decorated with AuNPs using the electrodeposition method. Then, glucose was deposited as an analyte on SERS substrates. Interestingly, the limit of detection (LOD) of SERS-active FTO-covered glass substrates (SERS-FGS) for the detection of glucose was 10−9 M. Density functional theory (DFT) method was used to simulate and validate the Raman spectra and peak signals obtained experimentally. Moreover, the average relative standard deviation (RSD) was calculated as 3.06% for ten repeated measurements. Furthermore, the enhancement factor for these SERS- based biosensors, for the identification of molecular vibrations of 10−5 M glucose, was obtained to be 1.4310 × 104 experimentally and 1.5397  × 104 numerically. Ther...
Source: Plasmonics - Category: Biomedical Science Source Type: research