Genes, Vol. 14, Pages 2177: Comparative Transcriptome Analysis of Galeruca daurica Reveals Cold Tolerance Mechanisms

Genes, Vol. 14, Pages 2177: Comparative Transcriptome Analysis of Galeruca daurica Reveals Cold Tolerance Mechanisms Genes doi: 10.3390/genes14122177 Authors: Hongling Zhang Feilong Sun Wenbing Zhang Xia Gao Lei Du Xiaopeng Yun Yanyan Li Ling Li Baoping Pang Yao Tan Galeruca daurica (Joannis) is a pest species with serious outbreaks in the Inner Mongolian grasslands in recent years, and its larvae and eggs are extremely cold-tolerant. To gain a deeper understanding of the molecular mechanism of its cold-tolerant stress response, we performed de novo transcriptome assembly of G. daurica via RNA-Seq and compared the differentially expressed genes (DEGs) of first- and second-instar larvae grown and developed indoors and outdoors, respectively. The results show that cold tolerance in G. daurica is associated with changes in gene expression mainly involved in the glycolysis/gluconeogenesis pathway, the fatty acid biosynthesis pathway and the production of heat shock proteins (HSPs). Compared with the control group (indoor), the genes associated with gluconeogenesis, fatty acid biosynthesis and HSP production were up-regulated in the larvae grown and developed outdoors. While the changes in these genes were related to the physiological metabolism and growth of insects, it was hypothesized that the proteins encoded by these genes play an important role in cold tolerance in insects. In addition, we also investigated the expression of genes related to the met...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research