Viruses, Vol. 15, Pages 2349: Development of an Immunochromatography Assay to Detect Marburg Virus and Ravn Virus
Viruses, Vol. 15, Pages 2349: Development of an Immunochromatography Assay to Detect Marburg Virus and Ravn Virus
Viruses doi: 10.3390/v15122349
Authors:
Katendi Changula
Masahiro Kajihara
Shino Muramatsu
Koji Hiraoka
Toru Yamaguchi
Yoko Yago
Daisuke Kato
Hiroko Miyamoto
Akina Mori-Kajihara
Asako Shigeno
Reiko Yoshida
Corey W. Henderson
Andrea Marzi
Ayato Takada
The recent outbreaks of Marburg virus disease (MVD) in Guinea, Ghana, Equatorial Guinea, and Tanzania, none of which had reported previous outbreaks, imply increasing risks of spillover of the causative viruses, Marburg virus (MARV) and Ravn virus (RAVV), from their natural host animals. These outbreaks have emphasized the need for the development of rapid diagnostic tests for this disease. Using monoclonal antibodies specific to the viral nucleoprotein, we developed an immunochromatography (IC) assay for the rapid diagnosis of MVD. The IC assay was found to be capable of detecting approximately 102−4 50% tissue culture infectious dose (TCID50)/test of MARV and RAVV in the infected culture supernatants. We further confirmed that the IC assay could detect the MARV and RAVV antigens in the serum samples from experimentally infected nonhuman primates. These results indicate that the IC assay to detect MARV can be a useful tool for the rapid point-of-care diagnosis of MVD.
Source: Viruses - Category: Virology Authors: Katendi Changula Masahiro Kajihara Shino Muramatsu Koji Hiraoka Toru Yamaguchi Yoko Yago Daisuke Kato Hiroko Miyamoto Akina Mori-Kajihara Asako Shigeno Reiko Yoshida Corey W. Henderson Andrea Marzi Ayato Takada Tags: Article Source Type: research
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